As a bioengineering laboratory with a specialization in microfluidic devices, we have identified a need for experiment-specific software-based tools to analyze the information rich imaging data obtained during novel in vitro microscopy-based assays, especially those that involve hematology applications and fluid flow. iCLOTS is a free, open-source cellular image analysis software augmented by machine learning designed for all biomedical researchers. iCLOTS processes your hard-won experimental data into detailed numerical and graphical analyses and interpretations.